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Received:December 08, 2016  Revised:February 22, 2017  Click here to download the full text
Citation of this paper:,2017..Sciences in Cold and Arid Regions,9(2):167~174.
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Author NameAffiliationE-mail
Constantine Uwaremwe School of Environmental and Municipal Engineering, Lanzhou Jiaotong University, 88 West Anning Road, Lanzhou, Gansu 730070, China  
ShiWeng Li School of Environmental and Municipal Engineering, Lanzhou Jiaotong University, 88 West Anning Road, Lanzhou, Gansu 730070, China lishweng@mail.lzjtu.cn 
XiMing Chen Key Laboratory of Extreme Environmental Microbial Resources and Engineering, Northwest Institute of Eco-Environment and Resource, Chinese Academy of Sciences, Lanzhou, Gansu 730070, China  
Maurice Ngabire School of Environmental and Municipal Engineering, Lanzhou Jiaotong University, 88 West Anning Road, Lanzhou, Gansu 730070, China  
Tawheed Mohammed Elheesin Shareef School of Environmental and Municipal Engineering, Lanzhou Jiaotong University, 88 West Anning Road, Lanzhou, Gansu 730070, China  
Juan Li School of Environmental and Municipal Engineering, Lanzhou Jiaotong University, 88 West Anning Road, Lanzhou, Gansu 730070, China  
MingHui Wu Key Laboratory of Extreme Environmental Microbial Resources and Engineering, Northwest Institute of Eco-Environment and Resource, Chinese Academy of Sciences, Lanzhou, Gansu 730070, China  
GuoLi Li School of Environmental and Municipal Engineering, Lanzhou Jiaotong University, 88 West Anning Road, Lanzhou, Gansu 730070, China  
基金项目:This study was financially supported by the National Natural Science Foundation of China (31400437, 31560121), the international cooperation program of Gansu (1504WKCA097), the application transformation foundation of CAS (HHS-CGZH-16-02) and UK BBSRC China Partnering Grant (BB/J020419/1).
 
Abstract:In this study, an Arthrobacter strain from desert soils in the Shule River Valley was isolated, China, which has a strong ability to convert cellulose to potential biofuel. In total, from five soil sample sites, six strains were isolated that grew well on CMC-Agar medium, with colony diameters ranging from 3~4 mm, among them, one strain had a strong ability to produce biofuels. Based on morphological and phylogenetic analyses, the isolate was identified as Arthrobacter nitroguajacolicus strain SLP1. The chemical properties of the biofuel extracted from the fermentation broth of strain SLP1 were analysed by gas chromatography and mass spectrometry. A total of 41 kinds of carbon compounds were identified, of those, five were detected at peak concentration and the carbon numbers ranged from C16- C22, which can be classified as alkanes, alkenes, and alcohols. Furthermore, biofuel-producing ability of strain SLP1 was enhanced using NTG mutagen. In a total of 94 mutant strains, four show the most enhanced biofuel production relative to the original strain. Biofuel production conditions were optimized by growing the four mutant strains on LB-Agar and SS-Agar medium.
keywords:Arthrobacter  cellulose  biofuels  mutagenesis
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